Journal: Molecular Biology
Article Title: Preventive Vaccination with Mesenchymal Stem Cells Protects Mice from Lethal Infection Caused by Herpes Simplex Virus 1
doi: 10.1134/S0026893321020242
Figure Lengend Snippet: Humoral immune response to immunization before and after infection of mice with lethal doses of HSV-1. (a) Titers of antiviral antibodies (ELISA); (b) relative levels of HSV-1-specific antibodies of IgG subclasses in immunized animals: group 1 (MSC), group 2 (MSCgD), group 3 (DNAgD), group 4 (iHSV), and group 5 (control). The results of ELISA are presented as absorbance values at 450 nm (OD 450 ). §Ratio IgG2a/IgG1. (c) Titers of neutralizing antibodies ( virus neutralization assay); * p < 0.05, between groups ( t -test); # p <0.05, compared to all groups ( t -test).
Article Snippet: The following test systems were used: Mouse IL-2 ELISA development kit (HRP), Mouse IL-6 ELISA development kit (HRP), Mouse IFNγ ELISA development kit (HRP), and Mouse TNF-α ELISA development kit (HRP) (Mabtech, Sweden).
Techniques: Infection, Enzyme-linked Immunosorbent Assay, Control, Virus, Neutralization